Saligrama Lab

Naresha Saligrama, PhD

The Saligrama lab is dedicated to understanding the adaptive immune responses in health and neurological disorders. Over the last few years it is becoming more and more evident that inflammation and adaptive immunity play a role in normal aging and neurological disorders. T cells are major component of the adaptive immune system and they recognize a diverse repertoire of antigens through T-cell receptors (TCRs). Major bottleneck in understanding T cell responses in health and disease is the diversity of TCRs and the vast variety of antigens that they can encounter. To circumvent these, we have taken a sequence-based approach to T cell responses and developed a robust single T cell TCR sequencing and phenotyping method and a bioinformatic analysis pipeline, which can group TCR sequences into clusters sharing specificity. Further, we have developed unbiased highthrouhput technologies to determine antigen specificity of TCRs. This is in contrast to traditional candidate approaches which involved a priori knowledge of the relevant antigens. By employing these technologies, we are exploring T cell responses at a “systems level” in varied neuroinflammatory, neurodegenerative, and neuropsychiatric disorders.

Principal investigator

Naresha  Saligrama, PhD

Naresha Saligrama, PhD

Assistant Professor, Neurology
Assistant Professor, Pathology and Immunology

  Saligrama lab

Recent publications

  • Cis-regulatory evolution of the recently expanded Ly49 gene familyFan, C., Xing, X., Murphy, S. J. H., Poursine-Laurent, J., Schmidt, H., Parikh, B. A., Yoon, J., Choudhary, M. N. K., Saligrama, N., Piersma, S. J., Yokoyama, W. M. & Wang, T., Dec 2024, In: Nature communications. 15, 1, 4839.Research output: Contribution to journal › Article › peer-review
  • A human STAT3 gain-of-function variant drives local Th17 dysregulation and skin inflammation in miceToth, K. A., Schmitt, E. G., Kolicheski, A., Greenberg, Z. J., Levendosky, E., Saucier, N., Trammel, K., Oikonomou, V., Lionakis, M. S., Klechevsky, E., Kim, B., Schuettpelz, L. G., Saligrama, N. & Cooper, M., Aug 5 2024, In: The Journal of experimental medicine. 221, 8Research output: Contribution to journal › Article › peer-review
  • An inducible genetic tool to track and manipulate specific microglial states reveals their plasticity and roles in remyelinationBarclay, K. M., Abduljawad, N., Cheng, Z., Kim, M. W., Zhou, L., Yang, J., Rustenhoven, J., Mazzitelli, J. A., Smyth, L. C. D., Kapadia, D., Brioschi, S., Beatty, W., Hou, J. C., Saligrama, N., Colonna, M., Yu, G., Kipnis, J. & Li, Q., Jun 11 2024, In: Immunity. 57, 6, p. 1394-1412.e8Research output: Contribution to journal › Article › peer-review
  • Influenza vaccine format mediates distinct cellular and antibody responses in human immune organoidsKastenschmidt, J. M., Sureshchandra, S., Jain, A., Hernandez-Davies, J. E., de Assis, R., Wagoner, Z. W., Sorn, A. M., Mitul, M. T., Benchorin, A. I., Levendosky, E., Ahuja, G., Zhong, Q., Trask, D., Boeckmann, J., Nakajima, R., Jasinskas, A., Saligrama, N., Davies, D. H. & Wagar, L. E., Aug 8 2023, In: Immunity. 56, 8, p. 1910-1926.e7Research output: Contribution to journal › Article › peer-review
  • KIR+CD8+ T cells suppress pathogenic T cells and ar active in autoimmune diseases and COVID-19Li, J., Zaslavsky, M., Su, Y., Guo, J., Sikora, M. J., van Unen, V., Christophersen, A., Chiou, S. H., Chen, L., Li, J., Ji, X., Wilhelmy, J., McSween, A. M., Palanski, B. A., Mallajosyula, V. V. A., Bracey, N. A., Dhondalay, G. K. R., Bhamidipati, K., Pai, J. & Kipp, L. B. & 16 others, Dunn, J. E., Hauser, S. L., Oksenberg, J. R., Satpathy, A. T., Robinson, W. H., Dekker, C. L., Steinmetz, L. M., Khosla, C., Utz, P. J., Sollid, L. M., Chien, Y. […]