Mingjie Li, MD, PhD, earned his MD from Jiamusi Medical College, China, in 1983 and a PhD from Kochi Medical School, Japan, in 1994. He worked at Yale University studying immunoglobulin gene recombination and subsequently at the Beckman Research Institute of the City of Hope, California, studying viral vector delivery of therapeutic genes against HIV infection before joining the Neurology Department at Washington University as an assistant research professor in April 2006.
Li serves as a senior scientist in the Viral Vectors Core at the Hope Center for Neurological Disorders. Gene delivery to nervous tissues is not only important for neuroscience research but also a potential means for the treatment of neurological disorders. However, most cell types relevant to nervous system disorders are difficult to transfect using non-viral transfection and traditional viral transduction methods. Genetic manipulation of brain cells in vivo has been even more challenging. Lentiviral vectors are able to transduce non-dividing cells, including neurons, making them attractive vehicles for gene delivery to neural cells. Several adeno-associated virus (AAV) serotypes have also been shown to successfully transduce cultured neurons and to mediate robust transgene expression in the brain.
We are developing new vectors for neurological applications. The goal of the Viral Vectors Core is to assist Washington University neuroscience researchers in the design and production of various kinds of vectors. Currently, we are focused on lentiviral and AAV vectors for use in cell culture and in vivo pre-clinical experiments designed to understand the causes and treatments for nervous system disorders. Among the projects we now undertaking are developing tissue-specific expression vectors and incorporating viral vectors with RNAi technology. We will also aim to promote translation of preclinical research findings into clinical applications.
- Generation of high-titer pseudotyped lentiviral vectorsHu, S., Li, M. & Akkina, R., 2019, Methods in Molecular Biology. Humana Press Inc., p. 125-134 10 p. (Methods in Molecular Biology; vol. 1937).Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review
- Gene therapy in neurological disordersLi, M. & Snider, B. J., Jan 1 2018, Elsevier. 428 p.Research output: Book/Report › Book › peer-review
- Gene therapy methods and their applications in neurological disordersLi, M. & Snider, B. J., Jan 1 2018, Gene Therapy in Neurological Disorders. Elsevier, p. 3-39 37 p.Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review
- Intracerebral adeno-associated virus gene delivery of apolipoprotein E2 markedly reduces brain amyloid pathology in Alzheimer's disease mouse modelsIntracerebral adeno-associated virus gene delivery of apolipoprotein E2 markedly reduces brain amyloid pathology in Alzheimer's disease mouse modelsZhao, L., Gottesdiener, A. J., Parmar, M., Li, M., Kaminsky, S. M., Chiuchiolo, M. J., Sondhi, D., Sullivan, P. M., Holtzman, D. M., Crystal, R. G. & Paul, S. M., Aug 1 2016, In: Neurobiology of Aging. 44, p. 159-172 14 p.Research output: Contribution to journal › Article › peer-review
- Correction to Potential role of orexin and sleep modulation in the pathogenesis of alzheimer's disease [The Journal of Experimental Medicine, 211, 13, (2014), 2487-2496]Correction to Potential role of orexin and sleep modulation in the pathogenesis of alzheimer's disease [The Journal of Experimental Medicine, 211, 13, (2014), 2487-2496]Roh, J. H., Jiang, H., Finn, M. B., Stewart, F. R., Mahan, T. E., Cirrito, J. R., Heda, A., Joy Snider, B., Li, M., Yanagisawa, M., de Lecea, L. & Holtzman, D. M., Jan 12 2015, In: Journal of Experimental Medicine. 212, 1, p. 121 1 p.Research output: Contribution to journal › Comment/debate
Mingjie Li, MD, PhD